Wpływ pasty z nanohydroksyapatytem na mikrotwardość powierzchni szkliwa z próchnicą początkową – badania in vitro

*Agnieszka Mielczarek¹, Joanna Michalik²

Summary
Introduction: Remineralization therapies based on various fluoride and nono-fluoride formulations have been proposed as useful in reversing the decay process in early caries.
Aim: The purpose of the study was the assessment of the effect of nanohydroxyapatite toothpaste on the surface of early caries lesions.
Material and methods: 90 polished human enamel specimens, embedded in cylindrical acrylic blocks served as a study material. The specimens were randomly assigned to 3 equinumerous groups, according to baseline microhardness of the enamel surface (SMH), assessed by Vickers method. The enamel specimens have been subjected to the preliminary demineralization, simulating the initial carious lesion. Subsequently, mean SMH were estimated. Upon demineralization one half of each specimen has been covered with a protective varnish. The exposed fragment has been subjected to the 3 week cycle of pH changes, according to the protocol as follows: NHAPF group – toothpaste containing nanohydroxyapatite (nano-HAP) and 1450 ppm F b.i.d., F group – toothpaste containing 1450 ppm b.i.d., K(-) group – distilled water application. After therapeutic pH – cycle process surface microhardness of samples was re-measured.
Results: Remineralization protocols implemented in NHAPF and F groups resulted in a comparable, statistically significant increase of SMH value from 49.52 to 189.35 VHN and from 50.25 to 164 VHN, respectively (p < 0.0001). In K(-) group further decrease of mean SMH value, below the level seen after demineralization, was observed (p < 0.01).
Conclusions: Comparable remineralization effect on carious enamel surface after application of both formulations was observed.

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