Techniki łączone oparte na spektrometrii mas we współczesnych badaniach klinicznych¹⁾

*Joanna Kałużna-Czaplińska, Wioletta Grys, Jacek Rynkowski

Summary
Coupled techniques have been widely applied in clinical research. They allow moniotring of metabolite concentrations in biological samples, such as body fluids, tissues and breath. Especially chromatography coupled with mass spectrometry is a very effective tool used to describe complex mixtures. Other the techniques used in clinical research are: gas chromatography-tandem mass spectrometry (GC-MS/MS), liquid chromatography-mass spectrometry (LC-MS), liquid chromatography-tandem mass spectrometry (LC-MS/MS), liquid chromatography-nuclear magnetic resonance-mass spectrometry (LC-NMR-MS) and capillary electrophoresis-mass spectrometry (CE-MS). These methods are applied in diagnostics and prognosis of diseases. Finding adequate biomarkers enables right diagnosis. Thanks to which early medical intervention is possible. Coupled techniques are used in the following studies: investigations of pharmacokinetics, pharmacodynamics, bioequivalence and bioavailability of medications, doping control and drug control, and clinical assestment of risk of occupational exposure to harmful substances. Thanks to those techniques the specificity and sensitivity of the analysis increase. They allow rapid and easy procedure, and in most cases do not require complicated sample pretreatment. They are more reliable and adequate for determination the trace concentrations in biological samples than classic biochemical investigations.

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