Wrażliwość na preparat Baikadent bakterii mikroaerofilnych wyizolowanych z blaszek miażdżycowych

*Anna Kędzia¹, Andrzej Kufel², Marek Ciecierski³ , Ewa Kwapisz¹, Maria Wierzbowska¹

Summary
The sensitivity to Baikadent (Herbapol, Wrocław) of 23 strains of microaerophilic bacteria isolated from carotid atherosclerotic plaques and 5 reference strains were tested. Investigation was carried out using the plate dilution technique in Brucella agar supplemented with 5% defibrynated sheep blood. Inoculum contained 10⁵ CFU per spot was seeded with Steers replicator upon the surface of agar with various Baikadent concentrations as well as upon that no Baikadent added (microaerophilic bacteria growth control). Incubation was performed in anaerobic jar (microaerophilic conditions, with Campy Pak, BBL) and reference of anaerobes strains in anaerobic conditions, at 37°C for 48 hrs. The MIC was defined as the lowest concentrations of the Baikadent that inhibited the growth of tested bacteria. The data showed, that the most susceptible to Baikadent (MIC ≤ 6.2 mg/ml) were the strains of Gram-positive rods Rothia dentocariosa and Rothia mucilaginosa. The strains of the genus Capnocytophaga gracilis and C. sputorum were sensitive in range ≤ 6.2-25.0 mg/ml. The least sensitive were the strains of Aggregatibacter actinomycetemcomitans. The growth approximately 29% and 57% of these strains was inhibited by concentrations of 12.5-25.0 mg/ml and 25.0-100.0 mg/ml. The results suggest that Baikadent could be useful for the supportive the periodontopathies prophylactic and therapy.

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