Otrzymywanie oraz dynamika wzrostu tkanki kalusowej rozwaru wielkokwiatowego – Platycodon grandiflorum Jacq. A. DC**

*Mariola Dreger, Małgorzata Górska-Paukszta, Anna Krajewska-Patan, Waldemar Buchwald

Summary
Balloon flower (Platycodon grandiflorum Jacq. A. DC) is a perennial plant (Campanulaceae family) originated from North-East Asia, traditionally used in bronchitis, asthma and cough. The roots of plant contain triterpenoids saponins – main active compounds with wide spectrum of pharmacology activities: expectoral, anti-inflammatory, hepatoprotective, anti-hyperlipidemic, cholesterol-lowering, and anticancer too. Studies on Platycodon grandiflorum in vitro cultures were limited so far to hairy root cultures, biotransformation and micropropagation protocols. The aim of the study was to obtain and determine growth parameters of callus tissue. Callus cultures were initiated from explants derived from sterile seedlings on solidified MS and LS medium supplemented with 2,4-D, NAA, BA and adenine chloride. Callus tissue obtained from root explants (line K) and cultured on MS medium with BA (2.0 mg/l), NAA (2.0 mg/l) and adenine chloride (1.0 mg/l) was chosen as material for growth experiments. Measurement of growth parameters (fresh and dried weight, content of dry weight and growth index) was performed in duplicate for 24 and 27 passages. Results indicated an intense growth of callus up to the 15^th day of culture, after which time tissue was growing slowly. The differences in increase of biomass between passages were noted. Callus (passage 24) was able to increase biomass (fresh weight) up to 7-fold, while passage 27 – 2-fold only. The results showed that callus tissue is characterized by good growth parameters and can provide adequate source of material for suspension culture and further research.

To jest tylko fragment artykułu. Aby przeczytać całość, przejdź do Czytelni medycznej.