Działanie preparatów Dentosept, Dentosept A i Salviasept na bakterie mikroaerofilne wyodrębnione z blaszek miażdżycowych tętnic szyjnych

*Anna Kędzia¹, Andrzej Kufel², Marek Ciecierski³, Joanna Wiśniewska³, Ewa Kwapisz¹, Maria Wierzbowska¹

Summary
The presence of the oral bacteria known for their role in periodontal infections detected in atherosclerotic plaques using different techniques (particularly of polymerase chain reaction technique – PCR). They are mainly Gram-negative rods such as Porphyromonas gingivalis, Prevotella intermedia, Fusobacterium nucleatum, Tannerella forsythia, Treponema denticola, Aggregatibacter actinomycetemcomitans, Campylobacter rectus and Eikenella corrodens. The herbal drugs are frequently administered prophylactically and for treatment oral cavity infections (eg. periodontal diseases). Among the drugs are Dentosept, Dentosept A and Salviasept. They exhibits anti-inflammatory and antimicrobial properties. The aim of this study was to determine the activity of Dentosept, Dentosept A and Salviasept against microaerophilic bacteria. The bacteria were isolated from carotid atherosclerotic plaques. The susceptibility of bacteria was determined by means of the dilution technique in Brucella agar supplemented with 5% sheep blood. The inoculum containing 10⁵ CFU per spot was applied to agar with herbal drug and without drug (strains growth control). Incubation was performed in anaerobic jar (microaerophilic conditions, with Camy Pak, BBL) and reference strains in anaerobic conditions, at 37°C for 48 hrs. The MIC was defined as the lowest concentrations of the herbal drug that inhibited the growth of tested bacteria. The data showed that the most susceptible to Dentosept, Dentosept A (MIC ≤ 0.3 mg/ml for 43% strains) and Salviasept (MIC ≤ 0.15 mg/ml for 71% strains) were the strains of Aggregatibacter actinomycetemcomitans. The strains belonging to the genus of Capnocytophaga ochracea were less sensitive to the drugs. MIC of the strains were in the ranges from 2.5 to > 20.0 mg/ml for Dentosept and Dentosept A and 0.6-5.0 mg/ml for Salviasept. The Salviasept was the more active against tested microaerophilic bacteria then Dentosept and Dentosept A.

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