Ocena właściwości cytotoksycznych etanolowego ekstraktu z propolisu w stosunku do komórek raka okrężnicy HCT 116

*Robert Kubina, Agata Kabała-Dzik, Beata Bielec, Katarzyna Smolarz, Barbara Stawiarska-Pięta, Ewa Szaflarska-Stojko

Summary
Numerous researches showed that propolis exhibits many healing properties. Not only has it antibacterial, anti-inflammatory, antioxidant, immunoprotective and hepatoprotective activities, but also anti-proliferative and cytotoxicity ones. The aim of this study was to evaluate total polyphenolic content and in vitro cytotoxic activity of ethanol extract of propolis depending on geographic location from where the propolis was derived and EEP concentrations used on HCT 116 colon cancer cells in in vitro tests.
Propolis samples were collected from different geographical locations in Poland. Propolis was characterized by polyphenolic content and flavonoids. A solution of ethanol extract of propolis in dimethyl sulfoxide was used as a material for the research. Ethanol from the extraction of propolis was removed by vaporization under vacuum. Cytotoxicity was evaluated by means of the MTT and LDH assays.
The cytotoxic effect of EEP at concentrations of 3.125-100 μg/ml after a 24-h incubation was from 1.11 % to 22.96% cell death. When EEP at concentration of 100 μg/ml was used, cytotoxicity amounted to 22.96%; 19.31% and 14.84% respectively for propolis from Kamianna, Łomża Landscape Park and Korbielów.
The results showed that in the case of the extract of propolis could inhibit cell growth of HCT 116 cell line. Ethanol extract of propolis from Poland obtained in the study exhibits cytotoxicity activity in colorectal carcinoma cells in vitro. MTT assay demonstrated that anti-proliferative properties of ethanol extract of propolis are dependent on the applied concentration and geographical locations.

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