Argumenty potwierdzające możliwość neo-oogenezy w jajnikach dorosłych samic

*Leopold Śliwa

Summary
The establishment of maximum reserve of the oogenetic cells in early fetal life and the lack of possibility of the multiply in postnatal period are widely known and proved in female mammalian reproductive biology. However, this statement was questioned by Johnson et al. (2004). According to Johnson, there are primordial germ cells in the ovarian envelope (tunica albuginea), which are able to undergo the mitotic divisions. Moreover, he postulates that oocytes undergo meiosis and mature in the formed ovarian follicles. This phenomenon was called a neo-oogenesis. The Johnson’s publication was the beginning of research on possibilities and efficiency of this process.
Currently there is a lot of scientific evidence for and against the possibilities of the postnatal oocytes formation and the ovarian follicles regeneration in the mammals and, what is more important, in the human. The results of the following research may confirm the neo-oogenesis hypothesis:
1. The morphological tests, in which the histological and histochemical methods as well as the genetic modified primordial germ cells are used.
2. 5-bromo-2-deoxyuridine (BrdU) incorporation and detection in mtDNA anti-BrdU antibody in germ cells located in the ovarian surface epithelium (OSE), which indicates on division activity of examined cells.
3. Cultured of cells derived from OSE gives rise to oocyte-like cells, genetics analysis and transplantation to adult female.
Busulphan-induced depletion of the follicle reserve and regeneration de novo of ovarian follicle.
Identification of germ cells and oocytes derived from bone marrow and peripheral blood macrophage and follicle regeneration.
The further research, proper interpretation of the results, wide discussion and extensive knowledge of the oogenesis course and control in the mammals and human are required to elucidate this problem.

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