Wrażliwość bakterii beztlenowych wyizolowanych z blaszek miażdżycowych tętnic szyjnych na Dentosept

*Anna Kędzia¹, Maria Wierzbowska¹, Andrzej Kufel², Marek Ciecierski²

Summary
The investigation of aetiology of atherosclerosis shows close relationships between inflammatory processes and the evolution of plaques. Chronic dental infections are strongly associated with atherosclerosis. Recent publication suggest connection between periodontitis and main periodontal bacterial pathogens (especially anaerobes) and atherosclerosis. Herbal drugs (e.g. Dentosept) are more and more frequently used in prophylactic and therapy of oral cavity infections. The aim of this study was evaluation the susceptibility of anaerobic bacteria to Dentosept. A total of 23 strains anaerobic bacteria isolated from carotid atherosclerotic plaques was tested. The susceptibility (MIC) of anaerobes was determined by means of plate dilution techniques, in Brucella agar, supplemented with 5% sheep blood. Inoculum containing 10⁵ CFU/spot was seeded with Steers replicator upon the surface agar. Incubation was performed in anaerobic yar in anaerobic conditions, at 37°C for 48 hrs. The MIC was interpreted as the lowest concentration Dentosept inhibiting the growth of anaerobic strains. The results indicated, that the strains from Gram-negative rods from genus of Tannerella forsythia were the most sensitive to Dentosept (MIC ≤ 0,3 mg/ml). The strains from genus of Porphyromonas gingivalis and Fusobacterium nucleatum were less sensitive to this herbal drug (MIC in ranges 0,6 -≥ 2,0 mg/ml). From among Gram-positive anaerobes the most sensitive were the strains Micrococcus micros (MIC < 0,3 mg/ml). The Gram-positive rods from genera of Propionibacterium and Actinomyces were less sensitive to Dentosept. MIC of the strains were the ranges from ≤ 0,3 to ≥ 2,0 mg/ml. The Dentosept was the more active against Gram-positive bacteria than Gram-negative rods.

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