Aktywność przeciwdrobnoustrojowa olejku z drzewa herbacianego (Tea Tree Oil) w badaniach in vitro. Cz. II

Aleksandra Garbusińska, *Anna Mertas, Ewelina Szliszka, Wojciech Król

Summary
Tea Tree Oil obtained from the Australian plant Melaleuca alternifolia (TTO – ang. Tea Tree Oil) is the one of the strongest antiseptic. The effects of treatment of infections with the use of TTO was confirmed with the clinical research. The pioneer of the research of the oil from Melaleuca alternifolia was Penfold, who in the twenties of XX^th century and later carried out the first experiments confirming the antiseptic properties of TTO by the comparison of the bactericidal activity of TTO with the butyric acid activity used as the disinfectant at that time. In this article we presented the examples of outcomes of the research of TTO activity against the reference strains of microorganisms and against the microorganisms isolated from the most frequent biological materials of the patients from different medical centres. For tested Gram-positive and Gram-negative bacteria, yeasts-like fungi, dermatophytes and filamentous fungi, the observed values of MIC TTO were low, also for drug-resistant strains. The antimicrobial activity of TTO is then high. The examples of the increase of the action of TTO combined with other antimicrobial substances were presented and this sy-nergistic effect was then measured using the disc diffusion test. The increase of inhibition zones around the paper disks impregnated with TTO combined with other substances in view of inhibition zones of around the paper discs impregnated with TTO alone was observed. Furthermore in the dilution methods the reduction of MIC TTO values in combination with other substances was observed when compared with the values of MIC TTO alone, using the indicator FICI to the evaluation of their changes. The curves time kill were also used for analysis of the speed of the microbicidal processes of combined substances. Synergistic activities were stated in case of TTO in combination with the other essential oils against staphylococci, TTO with amphotericin B against Candida strains, TTO with tobramycin against Escherichia coli and Staphylococcus aureus, TTO with copper lactate and TTO with copper lactobio-nate against Candida albicans and Lactobacillus acidophilus, TTO with chlorhexidine gluconate against Streptococcus mutans and Lactobacillus plantarum. In this article the cases of non-synergistic effect, or even the antagonism effect of TTO combined with other substances were also presented.

To jest tylko fragment artykułu. Aby przeczytać całość, przejdź do Czytelni medycznej.