Aktywność olejku pomarańczowego (Oleum Aurantii) wobec bakterii beztlenowych

*Anna Kędzia¹, Barbara Kochańska², Aida Kusiak³, Andrzej W. Kędzia⁴, Maria Wierzbowska¹, Ewa Kwapisz¹

Summary
A total of 33 strains of anaerobic bacteria isolated from infections of oral cavity were tested. The susceptibility (MIC) of anaerobes to orange oil (Avicenna-Oil, Wrocław) was determined by means of plate dilution technique in Brucella agar with 5% defibrinated sheep blood. Inoculum containing 10⁵ CFU/spot was seeded with Steers replicator upon the surface of agar with and without oil (strain growth control). The inoculated plates were incubated at 37°C for 48 h in anaerobic jars. The MIC was defined as the lowest concentration of the oil inhibiting visible growth of anaerobic strains. The results indicated that the most susceptible to tested essential oil from tested anaerobes were the Gram-negative rods from genus Bacteroides vulgates, Gram-positive cocci from genus Peptostreptococcus anaerobius and rods Propionibacterium acnes (MIC in ranges 5.0-7.5 mg/ml). The strains from the genera of Prevotella intermedia and Propionibacterium granulosum were the lowest sensitive to orange oil (MIC in ranges from 7.5 to 10.0 mg/ml). The MIC for 79% of all tested strains was in the concentration = 7.5 mg/ml.

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