Aktywność olejku cynamonowego (Oleum Cinnamomi) wobec bakterii beztlenowych

*Anna Kędzia

Summary
In the present study, cinnamon oil (Oleum Cinnamomi) were investigated for activity against 31 anaerobic bacteria isolated from oral infections. The susceptibility (MIC) anaerobes was determined by means of plate dilutions technique in Brucella agar with 5% sheep blood, menadione and hemin. The inoculum containing 10⁵ CFU/spot was seeded with the Steers inoculator upon the surface of agar. Incubation the agar plates was performed in anaerobic conditions at 37°C for 48 hrs. The MIC was interpreted as the lowest concentrations of cinnamon oil completely inhibiting the growth of bacterial strains. The results indicated that the most susceptible to tested oil from Gram-negative rods were the strains from the genera of Bacteroides (MIC = 0.25-0.5 mg/ml). However, remaining Gram-negative bacteria were slightly less susceptible to oil (MIC = 0.5-1.0 mg/ml). From among Gram-positive anaerobes the most sensitive were the cocci than rods (MIC ≤ 0.06-0.5 mg/ml). The concentrations ≤ 0.06 mg/ml inhibited the growth 57% of strains of cocci. The Gram-positive anaerobic bacteria were more sensitive to tested essential oil than Gram-negative.

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