Ocena działania olejku eukaliptusowego na bakterie tlenowe

*Anna Kędzia¹, Barbara Kochańska², Aida Kusiak³, Ewa Kwapisz¹, Maria Wierzbowska¹

Summary
This study was aiming to evaluate activity of eucalyptus oil on 31 aerobic bacteria isolated from patients with infections of oral cavity, respiratory tract, gastrointestinal tract and derma. Susceptibility (MIC) was determined two fold dilution method in Mueller-Hinton agar. The suspension of test organisms were 10⁶ CFU per spot and was seeded with Steers replicator upon the surface of agar containing various concentrations as well as upon that with no oil added (bacterial strains growth control). Incubation was performed in aerobic conditions at 37°C for 24 hours. MICs values were determined as the lowest concentrations that inhibited visible growth of the tested aerobic bacteria. The data show that the most susceptible to eucalyptus oil was Gram-positive cocci and rods. MIC for these strains was to the concentrations from ≤ 3.1 to 6.2 mg/ml. The Gram-negative rods were less sensitive (MIC=6.2 – ≥ 50.0 mg/ml). The strains from the genera of Pseudomonas were the lowest sensitive to eucalyptus oil (MIC in ranges 12.5 – ≥ 50.0 mg/ml). Gram-negative bacteria were more resistant to the tested essential oil than Gram-positive bacteria.

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