Evaluation Attempt of Bee Larva DNA Influence on the Course of Rat Pregnancy

*Agata Kabała-Dzik¹, Anna Rzepecka-Stojko², Jerzy Stojko³, Ewa Szaflarska-Stojko¹, Magdalena Wyszyńska¹, Robert Kubina¹, Aleksandra Kolarczyk¹, Wojciech Marquardt¹, Barbara Stawiarska-Pięta¹

Summary
Introduction. Beneficial properties of products collected, processed and secreted by bees, have been known and used for centuries. The latest trend in apitherapy is examination of a possibility to apply bee larva extract in proteinotherapy. The key to success and at the same time the greatest difficulty in experiments regarding a therapy with DNA application, is the use of an appropriate carrier, called vector. The simplest way of genetic material transport is a direct injection into target, uncovered tissues or organs, bare DNA molecules, suspended in a buffer solution.
The aim of the study. The aim of the study was evaluation of bee larva DNA influence upon the course of rat pregnancy, following the administration by intraperitoneal injection. Control group I included 10 rat females, which were administered sodium chloride solution by intraperitoneal injection on day 4, 10 and 14 of pregnancy. Experimental group I included 10 females, which were administered bee larva DNA biopreparation by intraperitoneal route in 50 μg on day 4, 10, 14 of pregnancy.
Material and methods. After the elimination of both animal groups on 21^st day of pregnancy, zootomic examinations of mothers and fetuses were performed, including: macroscopic inspection of females′ internal organs, evaluation of culture parameters of females, external inspection of rat fetuses and morphometric measurements of their bodies and evaluation of the number of ossification points in fetuses.
Results and conclusions. The analysis of the results obtained in both examined groups indicates, that bee larva DNA does not cause pathologic changes within internal organs of pregnant female rats, does not cause defects in fetuses′ development and also does not contribute to the occurrence of congenital defects in fetuses.

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