Działanie oleju kamforowego na bakterie beztlenowe

*Anna Kędzia¹, Andvzej W. Kędzia²

Summary
The sensitivity to camphor oil of 67 strains of anaerobic bacteria isolated from infections of oral cavity and respiratory tract were tested. The components of camphor oil was: camphora (10 parts) and rape oil (90 parts). The susceptibility of anaerobes was performed by means of plate dilution technique in Brucella agar with added 5% defibrynated sheep blood, menadione and hemin. The inoculum containing 10⁵ CFU per spot was seeded Steers replicator upon the surface of the agar with and without the oil (strains growth control). Incubation was performed at 37°C for 48 hrs in anaerobic jars under anaerobic conditions. The MIC was defined as the lowest oil concentrations that completely inhibited the growth of anaerobic bacteria. The results indicated that the most susceptible to camphor oil, from Gram-negative bacteria, were the strains from genera Prevotella and Porphyromonas. MIC´s for 42% and 40% of these strains were in the ranges Ł 0,12-0,5 mg/ml. The strains from the genera of Bacteroides and Veillonella were the lowest sensitive to tested oil (MIC in range of 1,0 – ł 4,0 mg/ml). The Gram-positive anaerobes were the most sensitive to camphor oil. The growth of 85% of these strains were inhibited by low concentrations within the range of Ł 0,12-0,5 mg/ml. The Gram-positive cocci were the more susceptible (90%) than Gram-positive rods (80%). The camphor oil was most effective against Gram-positive than Gram-negative anaerobes.

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