Działanie olejku cedrowego ( Oleum cedri) na bakterie beztlenowe

*Anna Kędzia

Summary
In this study, the susceptibility to cedar oil 75 strains of anaerobic bacteria were investigated by the plate dilution technique in Brucella agar supplemented with 5% sheep blood, menadione and hemin. The bacteria were isolated from patients with infections of oral cavity or respiratory tract. The inoculum containing 10⁵ CFU per spot was seeded with Steers replicator upon the surface of agar with oil and without oil (strains growth control). Incubation the plates was performed in anaerobic conditions in anaerobic jar in 37°C for 48 h. The MIC was defined as the lowest concentration of the cedar oil inhibiting the growth of anaerobes. The results showed, that the most susceptible to cedar oil in ranges Ł 0.06-0.25 mg/ml from Gram-negative rods were the strains from genus of Tannerella forsythia and Prevotella bivia (75% and 100% strains susceptible). The strains from the genera of Dialister, Bacteroides and Veillonella were the lowest sensitive to tested oil (MIC 1.0-ł3.0 mg/ml). The tested etheric oil was very active against Gram-positive cocci. MIC for 64% of the strains was to the concentration within the ranges from Ł 0.06 to 0.12 mg/ml. The Gram-positive cocci were the most sensitive than Gram-positive rods.

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