Działanie in vitro olejku sosnowego wobec bakterii beztlenowych wyizolowanych z jamy ustnej i dróg oddechowych

*Anna Kędzia¹, Andrzej W. Kędzia²

Summary
In the present study, pine oil (Oleum pini silvestris) were investigated for activity against 98 anaerobic bacteria using plate dilution technique method in Brucella agar. The inoculum contained 10⁶ CFU per spot. The agar plate were inoculated using a Steers replicator. Incubation the plater was performer in anaerobic jars in 37°C for 48 hrs. The MIC was interpreted as the lowest concentrations of pine oil completely inhibiting the growth of anaerobes. The results indicated that the most susceptible to tested aetheric oil from Gram-negative rods were the strains from the genera of Prevotella and Porphyromonas. MIC´s for 55% and 47% of these strains respectively, were to the concentrations within the range from ≤ 0.03 to 0.5 mg/ml. The strains from the genera of Bacteroides, particularly from species of B. fragilis were the lowest sensitive (MIC in ranges 0.25 ≥ 2.0 mg/ml). The strains of Gram-negative cocci from the genera of Veillonella were the most resistance (MIC 1.0-≥ 2.0 mg/ml). The Gram-positive rods and cocci showed similar susceptibility to pine oil. Lower concentrations in ranges ≤ 0.03-0.5 mg/ml inhibited the growth of 44 and 42% these bacteria respectively.

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