Ocena aktywności antybiotycznej nowozelandzkiego miodu manuka metodą rozcieńczeń seryjnych w podłożu płynnym

*Elżbieta Hołderna-Kędzia¹, Henryk Ostrowski-Meissner², Bogdan Kędzia¹

Summary
In the studies has been compared the antibiotic activity declared by producer of manuka honey samples using the well method with phenolic equivalent, with their activity determined by the method of serial dilutions in liquid medium. It were investigated 50 samples of manuka honey from New Zealand and Australia. The samples were been divided in 5 groups: 13 samples with nondetermined of UMF value (group A), 13 samples with the value UMF 5+ (group B), 12 samples with the value UMF 10+ (group C) also 7 and 5 samples with the values respectively 15+ (group D) and 20+ (group E). The mean inhibine values obtained for samples of each groups were: 2.7 (group A), 3.0 (group B), 3.1 (group C), 3.5 (group D) and 3.8 (group E).
It was shown that the manuka honey samples belonging to groups A, B, and C were less antibiotic active (inhibine value ≤3), than samples belonging to groups D and E (inhibine value>3). The statistic studies shown, that between those groups of honey exists the statistic dependence (p<0.0001). Moreover basing on carried out comparative studies of methods of determination of antibiotic activity of manuka honey samples, the dependence between results obtained by determination of UMF values and estimation of inhibine value is not confirmed.

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