Działanie olejku z mięty pieprzowej ( Oleum menthae piperitae) na bakterie beztlenowe

*Anna Kędzia

Summary
The sensitivity to peppermint oil (Avicenna-Oil) 65 strains of anaerobic bacteria isolated from patients with infection of oral cavity and respiratory tract were tested. Susceptibility (MIC) anaerobes was determined by means of plate dilution technique in Brucella agar supplemented with 5% defibrynated sheep blood, menadione and hemin. The inoculum containing 10⁶ CFU per spot was seeded with Steers replicator upon the surface of agar containing various oil considerations as well as upon that with no oil added (strains growth control). Incubation the plates was performed in anaerobic conditions in anaerobic jars in 37°C for 48 hours. The MIC was defined as the lowest concentration of the oil inhibiting the growth of the strains of anaerobic bacteria. The results indicated that the most susceptible to tested aetheric oil from Gram-negative rods were the strains from the genera of Porphyromonas. MIC for 70% of these strains was to the concentrations within the from 0.5 to ≤ 0.12 mg/ml. The strains of Bacteroides and Tannerella were the lowest sensitive (MIC in ranges 2 -≥ 4 mg/ml). The oil was very active against the all Gram-positive anaerobes. The concentrations in ranges from ≥ 0.12 to 0.5 mg/ml inhibited the growth of 100% the cocci and 90% the rods.

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