Działanie olejku z drzewa herbacianego na bakterie beztlenowe wyhodowane z zakażeń dróg oddechowych

Anna Kędzia¹, Henry Ostrowski-Meissner², Andrzej W. Kędzia³

Summary
The sensitivity to tea tree oil (Australian Company TTD International Pty Ltd, Sydney) of 138 strains of anaerobic bacteria isolated from patient with infections of respiratory tract were tested. The susceptibility (MIC) anaerobes were determined by means of plate dilution technique in Brucella agar supplemented with 5% defibrynated sheep blood, menadione and hemin. Inoculum containing 10⁶ CFU per droplet was seeded with Steers replicator upon the surface of agar containing various oil concentrations as well as upon that with no oil added (strains growth control). Incubation the plates were performed at 37°C in anaerobic jars in anaerobic conditions for 48 hrs. The MIC was defined as the lowest concentration of the oil inhibiting the growth of the strains anaerobic bacteria.The results indicated that the most susceptible to oil were the strains Gram-negative anaerobes belonging to the genera Fusobacterium and Veillonella. The MICs for 64-60% of these strains were in ranges Ł 0.03-0.5 mg/ml. Among Gram-positive anaerobic bacteria the strains from genera of Actinomyces and anaerobes streptococci were the most sensitive. The growth all of these strains (100%) were inhibited by tea tree oil within the range of Ł 0.03-0.06 mg/ml. The strain of Eubacterium lentum was the lowest sensitive to tested oil (MIC = 2 mg/ml). The growth of 100-67% of these strains were inhibited by tea tree oil within the range of Ł 0.03-0.5 mg/ml. The strain of Eubacterium lentum was the lowest sensitive to tested oil (MIC = 2 mg/ml).

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