Ocena działania przeciwbakteryjnego olejku goździkowego ( Oleum Caryophylli)

*Anna Kędzia

Summary
The sensitivity to clove oil (Avicenna-Oil) 68 strains of anaerobic bacteria isolated from patients with infections of oral cavity was determined by means of plate dilution technique in Brucella agar supplemented with 5% defibrynated sheep blood, menadione and hemin. Inoculum containing 10⁶ CFU per spot was seeded with Steers replicator upon the surface of agar with oil and the plate without oil (strains growth control). Incubation the plates was performed in anaerobic conditions in anaerobic jar for 48 h in 37°C. The MIC was defined as the lowest concentration of the clove oil inhibiting the growth of anaerobes. The results of investigations indicated, the most sensitive to oil from Gram-negative rods were the strains from the genera of Fusobacterium. MIC for 50% of these strains were to the concentrations within the ranges from ≤0.06 do 0.5 mg/ml. The strains from the genera of Porphyromonas were the lowest sensitive to oil. The growth of the strains were inhibited by concentrations within the ranges of 0.5-≥2.0 mg/ml. The oil was very active against the Gram-positive anaerobic bacteria. The concentrations in ranges ≤0.06-0.5 mg/ml inhibited the growth of 62% the cocci and 57% the rods.

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