Ocena wrażliwości bakterii beztlenowych na olejek tymiankowy

*Anna Kędzia

Summary
A total of 88 strains of anaerobic bacteria isolated from samples obtained from 31 patients with periodontitis, gingivitis or infections of respiratory tract were tested. The susceptibility (MIC) anaerobes to thyme oil (Avicenna-Oil, Wrocław) was determined by means of plate the plate dilution technique in Brucella Agar supplemented with 5% defibrynated sheep blood, menadione and hemin. Inoculum containing 10⁶ bacteria (CFU) per spot was seeded with Steers replicator upon the surface of agar containing various oil concentrations as well as upon that with no oil added (bacteria growth control). Incubation were performed in anaerobic jars containing mixture of gases: 10% CO₂, 10% H₂ and 80% N₂, palladium catalyst and indicator of anaerobiosis, for 48 hs at 37°C. MIC was interpreted as the lowest concentration of essential oil inhibiting the growth of anaerobic bacteria.
The studies have been showed that Gram-negative rods from genera Dialister (100%), Bacteroides (58%), Prevotella (50%) and Gram-positive cocci from genera Anaerococcus (100%) and Peptoniphilus (100%) were the most susceptible to thyme oil (MIC in ranges ≤0,06-0,5 mg/ml). The strains from genera Porphyromonas and Finegoldia were the lowest sensitive to essential oil. Growth the strains were inhibited at a concentration of 1,0-≥2,0 mg/ml. Moreover, the Gram-positive anaerobic bacteria were slightly more sensitive to thyme oil than the Gram-negative.

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