Problem występowania pałeczek Legionella w instalacjach i urządzeniach wytwarzających aerozol wodno-powietrzny w obiektach służby zdrowia w Polsce

Renata Matuszewska, *Bożena Krogulska

Streszczenie
Aim:The aim of the study was to determine the frequency of L. pneumophila colonization in hot water systems and water-air aerosol producing devices in inpatient healthcare facilities in Poland.
Material and methods: Water samples were examined according to own NIH method and International Standard Method PN ISO 11731-2:2006. Water samples were concentrated by membrane filtration and cultured after low pH treatment on selective GVPC medium. Plates were incubated at 36±2^oC for up to 7 days and colonies with typical morphology were counted. Presumptive positive Legionella colonies were subsequently plated on medium without cysteine. The L-cysteine dependent strains were confirmed by latex agglutination (Legionella Latex Test).
Results: A total of 169 water samples were collected. L. pneumophila was isolated from 132 samples (78,7%). In 72,8% of positive samples the number of isolated L. pneumophila organisms exceeded the limit set in the Minister of Health Order of 29 March 2007 (O.J. 07.61.417) and ranged from 1,1x10² jtk/100 ml to 1,3x10⁵ jtk/100 ml. The most frequently isolated serogroups included 2-14, but the serogroup 1 was also detected (14,4% of samples).
Conclusions: Legionella frequently colonizing hot water systems and water-air aerosol producing devices in inpatients healthcare facilities is a potential cause of severe infection in hospitalized patients. Regular monitoring of hot water quality with periodic sampling and testing for the presence of legionella bacteria is necessary, as well as routine inspection of the water system condition and performance.

To jest tylko fragment artykułu. Aby przeczytać całość, przejdź do Czytelni medycznej.