SIGNIFICANCE OF AgNOR MEASUREMENT IN RAT THYROID GLAND

*Ewa Ogłodek¹, Danuta Mo?², Aleksander Araszkiewicz¹

Summary
Aim. The aim of this study was to evaluate the influence of extremely low-frequency magnetic fields with parameters used in the treatment of people in order to alleviate the pain and stimulate metabolism of cells in the activity of nucleoli and nucleolar organizers in female rat thyrocytes.
Material and method. 24 female Wistar rats, Hannover substrain, were included in the experiment. The rats were divided into 2 groups: group ”40”, exposed to extremely low-frequency magnetic fields of frequency 40 Hz; and control group ”C” with sham exposure. After taking the preparations out of water they were stained in a solution of silver nitrate, and again rinsed in water, and after that they were put, one by one, in 5% alkaline solution of sodium thiosulphate.
Results. In the preparations of rat thyroid glands the following parameters were evaluated: the number of nucleoli (NNU), the surface area of nucleoli (SANU) and the number of AgNOR nucleolar organizers (NAgNOR), and the surface area of AgNOR nucleolar organizers (SAAgNOR).
Conclusions. The observed changes of surface area and the number and surface areas of nucleoli and nucleolar organizers point to the increase of the replicative activity in nuclei of thyrocytes and possible increase of DNA synthesis.

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