Flow cytometric analysis of eosin-5-maleimide binding to protein of band 3 and Rh-related proteins in diagnosis of hereditary spherocytosis

*Jolanta Kłopocka

Summary
Introduction
Flow cytometric analysis of eosin-5-maleimide (EMA) binding to red cells is a screening test for the diagnosis of hereditary spherocytosis (HS).
Interaction of EMA with the ĺ-NH2 group of lysine in band 3 protein was previously reported.
Aim
The aim of this work was to introduce modified flow cytometric analysis of eosin-5-maleimide binding to protein of band 3 and Rh-related proteins as the basis of a screening test in diagnosis of hereditary spherocytosis.
Materials and methods
Red cells from 12 patients with membrane deficiencies in HS (spectrin in 4 patients, band 3 in 3 patients, protein 4.2 in 2 patients and combined spectrin/ankyrin in 3 patients) and from 26 healthy volunteers.
Flow cytometric analysis of chemically modified EMA-labelled red cells.
Results
Fluorescence intensity of EMA-labelled HS red cells is sensitive to changes
in the relative amounts of band 3, Rh AG, CD 47 and Rh proteins.
Conclusions
Binding of EMA to the integral proteins (band 3, RhAG, CD 47 and Rh proteins) implicated in red cell cytoskeleton underpins the high specificity of the flow cytometric test for HS

To jest tylko fragment artykułu. Aby przeczytać całość, przejdź do Czytelni medycznej.