Effect of perindopril on plasma carbonyl groups and the level of nitric oxide in elderly patients with essential hypertension

Kornelia Kedziora-Kornatowska¹, Hanna Pawluk², Tomasz Kornatowski³, Leszek Szadujkis-Szadurski³, Robert Pawluk², Jolanta Czuczejko², Jadwiga Motyl¹, Jozef Kedziora²

Summary
Introduction: The aim of the study was the measurement of the levels of nitric oxide and carbonyl groups in the plasma of elderly patients with essential hypertension.
Material and methods: Research was done on a control group of 18 healthy patients, and a group of 32 with essential hypertension patients. Prior pharmacological treatment, in case it was used, was stopped 2 days before the research was done. Then all of the patients with primary hypertension were applied perindopril in 4 mg/day dose for the period of 6 weeks. Patients with hypertension were tested after one week and 6 weeks since the start of perindopril therapy. Carbonyls formed by oxidation were determined by the Levine method.
The concentration of proteins in serum was analyzed using the burette method.
The nitrite concentration in plasma was analyzed by the Griess reaction according to Marlett et al.
Results: Hypertensive patients presented a statistically significant (p <0.001) higher carbonyl group concentration both before the therapy (0.25 ± 0.07 nmol/mg protein), and after 7 and 42 days (respectively 0.2 ± 0.08; 0.25 ± 0.07 nmol/mg protein) of perindopril application versus the control group (0.09 ± 0.02 nmol/mg protein). Hypertensive patients showed a lower NO level (1.47 ± 0.63 ?mol/l) compared to the control group (1.91 ± 0.60 ?mol/l), but this difference was not statistically significant.
Conclusions: The results show an increase in oxidative stress, and dysfunction of the vascular endothelium in eldery patients with hypertension

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