Ocena właściwości proapoptotycznych etanolowego ekstraktu propolisu w badaniach in vitro**

*Robert Kubina¹, Agata Kabała-Dzik¹, Rafał Jakub Bułdak², Ewa Szaflarska-Stojko¹, Paweł Tylka¹, Beata Bielec¹, Barbara Stawiarska-Pięta¹

Summary
Modern epidemiological data prove that the majority of substances of natural origin demonstrating anticancerogenic properties are non-toxic, safe and in a longer term do not influence a human organism in a negative way. The research involved an in vitro model with the use of propolis that belongs to these substances in order to show its anti-neoplastic properties. A solution of ethanol extract of propolis (EEP) in dimethyl sulfoxide was used as a material for the research. In the conducted research, an increase in the percentage of apoptotic cells in both the case of Me45 malignant melanoma and Hct-116 colorectal cancer cell lines was observed. Used EEP with concentration of 50 μg/ml caused a 16.56% increase in the number of apoptotic cells in the case of Me45 and a 21.02% increase in the case of Hct-116. A 0.498 ng/mg increase in the concentration of caspase-8 in the Me45 malignant melanoma cell culture, to which EEP with concentration 50 μg/ml was added, was showed in relation to its concentration in the control culture. In the case of Hct-116 line, an increase of caspase concentration amounted to 0.884 ng/mg in cultures with addition of the same dose of propolis, in relation to the value in the group without propolis. The increase in apoptosis of neoplastic cell lines in vitro was higher after administration of EEP with concentration of 100 μg/ml vs. 50 μg/ml.

To jest tylko fragment artykułu. Aby przeczytać całość, przejdź do Czytelni medycznej.